|The RAD9A rad9a (Catalog #MBS9215531) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAD9A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RAD9A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).
WB ~~ 1:500
IP ~~ 1:500. Researchers should empirically determine the suitability of the RAD9A rad9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RAD9A rad9a product has the following accession number(s) (GI #342672046) (NCBI Accession #NP_001230153.1) (Uniprot Accession #Q99638). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAD9A Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of RAD9A in Hela,MCF7,3T3,COS7 and Rat testis cell lysates using RAD9A mouse mAb (1:500 diluted).Predicted band size:43KDa.Observed band size:55KDa.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela and 3T3 cell lysates using RAD9A mouse mAb.)
Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3\'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3\'->5\' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Antigen Source: Human. Antigen Type: Recombinant Protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RAD9A are readily searchable from our website. Different antibodies against the same target such as RAD9A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.