|The RBFOX3 rbfox3 (Catalog #MBS9131507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RBFOX3 Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RBFOX3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:100 - 1:500
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the RBFOX3 rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RBFOX3 rbfox3 product has the following accession number(s) (GI #322510139) (NCBI Accession #A6NFN3.4) (Uniprot Accession #A6NFN3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RBFOX3 Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of U-87MG cells, using RBFOX3 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using RBFOX3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse cerebellum using RBFOX3 antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of SH-SY-5Y cells using RBFOX3 antibody.)
Immunogen: Recombinant protein of human RBFOX3. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RBFOX3 are readily searchable from our website. Different antibodies against the same target such as RBFOX3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Cleft Lip, Cleft Palate, Neurodegenerative Diseases are some of the diseases may be linked to RBFOX3 Monoclonal Antibody. Brain, Embryonic Tissue, Eye, Heart tissues are correlated with this protein.