anti-RELA antibody product blog
Tags: Antibody; anti-RELA antibody; RELA; Monoclonal Antibody;
The RELA rela (Catalog #MBS9131093) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RELA Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RELA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IP: 1:20 - 1:50. Researchers should empirically determine the suitability of the RELA rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RELA rela product has the following accession number(s) (GI #62906901) (NCBI Accession #Q04206.2) (Uniprot Accession #Q04206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RELA Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RELA antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (MBS128212) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
NF-kappa-B is an ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.
Immunogen: Recombinant protein of human RELA. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RELA are readily searchable from our website. Different antibodies against the same target such as RELA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Heart, Intestine, Kidney, Liver, Lung, Muscle, Vascular tissues are correlated with this protein. RELA also interacts with the following gene(s): CHUK, COMMD1, CREBBP, EP300, HDAC1, IKBKB, NFKB1, NFKBIA, NFKBIB, UBC.