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anti-RET antibody product blog

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Posted on 2017-05-29 21:50:45 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; RET; anti-RET antibody;
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The RET ret (Catalog #MBS9131777) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RET Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RET can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the RET ret for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RET ret product has the following accession number(s) (GI #547807) (NCBI Accession #P07949.3) (Uniprot Accession #P07949). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RET Monoclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using RET antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (MBS128212) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.)
Western Blot (WB) RET.

This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed.

Immunogen: A synthetic peptide of human RET. Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RET are readily searchable from our website. Different antibodies against the same target such as RET may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. RET also interacts with the following gene(s): CBL, CRK, DOK4, DOK5, DOK6, FRS2, GDNF, GFRA1, GRB2, SHC1. The following patways have been known to be associated with this gene.
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