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anti-RFXANK antibody product blog

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Posted on 2015-08-13 19:52:17 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; RFXANK; anti-RFXANK antibody;
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The RFXANK rfxank (Catalog #MBS832055) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RFXANK antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RFXANK can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB).
FC: 1:100
IHC: 1:150
IF: 1:100
WB: 1:500. Researchers should empirically determine the suitability of the RFXANK rfxank for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RFXANK rfxank product has the following accession number(s) (GI #3820616) (NCBI Accession #AAC69883.1) (Uniprot Accession #O14593). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RFXANK antibody with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Jurkat cells using RFXANK antibody (Red), compared to a nonspecific negative control antibody (Blue).)
Flow Cytometry (FC/FACS) RFXANK.

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant RFXANK protein (Right) or empty vector (Left) detected with RFXANK antibody)
Western Blot (WB) RFXANK.

Immunohistochemistry (IHC) (Immunohistochemical analysis of RFXANK protein in paraffin embedded Human tonsil tissue using RFXANK antibody)
Immunohistochemistry (IHC) RFXANK.

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant RFXANK protein using RFXANK antibody)
Immunofluorescence (IF) RFXANK.

Mouse monoclonal RFXANK antibody.

Biological Significance: Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Immunogen: RFXANK antibody was raised in Mouse using full length human recombinant protein of human RFXANK produced in HEK293T cell as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RFXANK are readily searchable from our website. Different antibodies against the same target such as RFXANK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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