anti-RHO antibody product blog
Tags: Antibody; Monoclonal Antibody; Rhodopsin; anti-RHO antibody; RHO;
The RHO rho (Catalog #MBS802096) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rhodopsin Antibody: Biotin reacts with Vertebrates and may cross-react with other species as described in the data sheet. MyBioSource\'s Rhodopsin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA), Immunofluorescence (IF).1:1000 (WB). Researchers should empirically determine the suitability of the RHO rho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RHO rho product has the following accession number(s) (GI #62460472) (NCBI Accession #NP_001014890.1) (Uniprot Accession #P02699). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rhodopsin Antibody: Biotin with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.)
Western Blot (WB) (Western Blot analysis of Human Cell lysates showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 1D4. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects ~40kDa band corresponding to the molecular weight of Rhodopsin. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin\'s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
Conjugate: Biotin. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide.