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anti-RHO antibody product blog

Posted on 2015-09-01 09:19:58 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; Rhodopsin; anti-RHO antibody; RHO;
The RHO rho (Catalog #MBS804415) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rhodopsin Antibody: ATTO 680 reacts with All mammalian species (Human, Mice, Rat, Bovine, Pig etc.), most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates. and may cross-react with other species as described in the data sheet. MyBioSource\'s Rhodopsin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB), ELISA (EIA).
1:1000 (WB), 1-2ug per 100-500ug of protein (IP). Researchers should empirically determine the suitability of the RHO rho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RHO rho product has the following accession number(s) (GI #62460472) (NCBI Accession #NP_001014890.1) (Uniprot Accession #P02699). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rhodopsin Antibody: ATTO 680 with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain. Localization: Staining of photoreceptor outer segment (OS). Other layers of the retina: IS - inner segment; ONL - outer nuclear layer; OPL - outer plexiform layer; INL - inner nuclear layer; IPL - inner plexiform layer; GCL - ganglion cell layer.)
Immunohistochemistry (IHC) RHO.

Background Info: Detects ~40kDa band corresponding to the molecular weight of Rhodopsin. Binds specifically to the N-terminus of Rhodopsin.

Scientific Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin\'s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Conjugate: ATTO 680. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RHO are readily searchable from our website. Different antibodies against the same target such as RHO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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