anti-RING1 antibody product blog
Tags: Antibody; Monoclonal Antibody; Ring1; anti-RING1 antibody;
The RING1 ring1 (Catalog #MBS608046) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RING1 (Polycomb complex protein RING1, ring finger protein 1, E3 ubiquitin-protein ligase RING1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RING1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: 0.5-2ug/ml
Immunocytochemistry: 1:500. Researchers should empirically determine the suitability of the RING1 ring1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RING1 ring1 product has the following accession number(s) (GI #296064) (Uniprot Accession #Q06587). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RING1 (Polycomb complex protein RING1, ring finger protein 1, E3 ubiquitin-protein ligase RING1) with the following immunoassay(s):
Testing Data (Confocal fluorescent analysis of NIH/3T3 and A431 cells using anti-RING1, MBS608046 (Red). Actin filaments have been labeled with AlexaFluor® 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive nuclear staining.)
Western Blot (WB) (Western Blot Analysis: Representative lot data. HEK293 cell lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-RING1, MBS608046 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates RING1 (~42kD).)
RING1 belongs to the RING finger family, members of which are characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain.
Immunogen: GST-tagged recombinant protein corresponding to RING1. Positive Control: HEK293 cell lysate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RING1 are readily searchable from our website. Different antibodies against the same target such as RING1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.