anti-RPA2 antibody product blog
Tags: Antibody; Monoclonal Antibody; RPA2; anti-RPA2 antibody;
The RPA2 rpa2 (Catalog #MBS603767) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RPA2 (Replication Protein A2, 32kD) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RPA2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry.
Dilution: Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2-50ug/ml. Researchers should empirically determine the suitability of the RPA2 rpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RPA2 rpa2 product has the following accession number(s) (GI #47496643) (Uniprot Accession #P15927). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RPA2 (Replication Protein A2, 32kD) with the following immunoassay(s):
Testing Data
Western Blot (WB) (Detection of human RPA2 by Western blot. Samples: Whole cell lysate (25 ug) from HeLa cells. Predicted molecular weight: 29 kD)
Immunoprecipitation (IP) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-RPA2 mAb.)
Testing Data (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-RPA2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor® 488- conjugated goat anti-mouse IgG.)
Immunostaining (IS) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-RPA2 mAb.)
RPA2 is required for DNA recombination, repair and replication. The activity of RP-An is mediated by single-stranded DNA binding and protein interactions.
Immunogen: Partial sequence of recombinant full-length protein to human RPA2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RPA2 are readily searchable from our website. Different antibodies against the same target such as RPA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bladder, Blood, Brain, Ganglia, Intestine, Kidney, Lymph, Muscle, Parathyroid, Skin tissues are correlated with this protein. RPA2 also interacts with the following gene(s): MEN1, PRKDC, RPA1, RPA3, UNG, XPA.