anti-RPA70/RPA1 antibody product blog
Tags: Antibody; Monoclonal Antibody; RPA70/RPA1; anti-RPA70/RPA1 antibody;
The RPA70/RPA1 rpa1 (Catalog #MBS476285) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-RPA70/RPA1 Mouse mAb reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s RPA70/RPA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (ICH), Immunocytochemistry (ICC), Flow Cytometry (FC).WB: 1/500-1/2000
IHC: 1/200-1/1000
ICC: 1/200-1/1000
FCM: 1/200-1/400
ELISA: 1/10000. Researchers should empirically determine the suitability of the RPA70/RPA1 rpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RPA70/RPA1 rpa1 product has the following accession number(s) (GI #4506583) (NCBI Accession #NP_002936.1) (Uniprot Accession #P27694). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-RPA70/RPA1 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of RPA70/RPA1 in Raji, HCT116, Jurkat, MCF7 and Hela cell lysates using RPA70/RPA1 mouse mAb (dilution 1:1000).Predicted band size:68kDa.Observed band size:70kDa.)
Western Blot (WB) (Western blot analysis using RPA1 mouse mAb against HeLa (1), MCF-7 (2), K562 (3), A431 (4), and COS-7 (6) cell lysate.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using RPA1 mouse mAb (green). Red)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of Jurkat cells using RPA1 mouse mAb (green) and negative control (red).)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded colon cancer tissues using RPA1 mouse mAb with DAB staining.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded prostate tissues using RPA1 mouse mAb with DAB staining.)
Entrez Summary: This gene Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing
UniPort Summary Function: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage (PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response (PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage (PubMed:17765923). Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair (PubMed:7697716). Plays also a role in base excision repair (BER) probably through interaction with UNG (PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance (PubMed:17959650). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange (PubMed:19996105).