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anti-rSec8 antibody product blog

Posted on 2017-05-19 00:02:26 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; rSec8; anti-rSec8 antibody;
The rSec8 exoc4 (Catalog #MBS565749) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The rSec8 monoclonal antibody (14G1) reacts with Human, Mouse, Rat, Sheep, Dog, Pig, Monkey, Bovine, Chicken, Fish, Guinea pig, Hamster, Rabbit and may cross-react with other species as described in the data sheet. MyBioSource\'s rSec8 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB).
Immunoprecipitation (12.5ug/ml), Western Blot (1:1\'000, colorimetric).
Detects a band of ~110kDa by Western blot. Researchers should empirically determine the suitability of the rSec8 exoc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The rSec8 exoc4 product has the following accession number(s) (GI #24418659) (NCBI Accession #Q62824.1) (Uniprot Accession #Q62824). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the rSec8 monoclonal antibody (14G1) with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis of human brain, cerebellum tissue stained with rSec8, mAb (14G1) at 5µg/ml.)
Immunohistochemistry (IHC) rSec8.

Western Blot (WB) (Western blot analysis of rSec8: Lane 1: MWM, Lane 2: Brain (rat), (tissue extract), Lane 3: Brain (mouse), (tissue extract), Lane 4: MDBK Cell Lysate.)
Western Blot (WB) rSec8.

rSec6 and rSec8 are rat homologs of the yeast Sec6 and Sec8 proteins that are essential for protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec8 is part of a 19.5S complex that includes Sec6 and Sec15, that also associate with the ras-like GTP binding protein Sec4. An analogous 17S complex in rat brain was found to contain rSec6 and rSec8, along with eight proteins with a combined molecular weight of 743 kDa. The membrane-associated rSec6/8 complex co-immunoprecipitates with syntaxin, providing evidence that this complex is involved in synaptic vesicle docking and fusion at the presynaptic terminal.

Immunogen: Recombinant rat rSec8. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing rSec8 are readily searchable from our website. Different antibodies against the same target such as rSec8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Colon, Liver, Lung, Prostate tissues are correlated with this protein.
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