|The RT1.Bu n/a (Catalog #MBS520418) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat RT1.Bu FITC (Clone OX-3) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RT1.Bu can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the RT1.Bu n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Rat RT1.Bu FITC (Clone OX-3) (mouse IgG1) with the following immunoassay(s):
anti-rat RT1.Bu monoclonal antibody recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains (1,2). This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). MBS520418 recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, MBS520418 is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras (3,4).
Immunogen: Rat thymocyte membrane glycoprotein
Donor: BALB/c spleen
Fusion Partner: P3-NS1-1-Ag4 (NS1/1). Presentation: 100 ug or 500 ug FITC conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RT1.Bu are readily searchable from our website. Different antibodies against the same target such as RT1.Bu may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.