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anti-S100A10 antibody product blog

Posted on 2017-05-26 04:43:15 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; S100A10; anti-S100A10 antibody;
The S100A10 s100a10 (Catalog #MBS475582) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-S100A10 Mouse mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s S100A10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF).
WB: 1:1000
ICC: 1:200
IP: 1:100. Researchers should empirically determine the suitability of the S100A10 s100a10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The S100A10 s100a10 product has the following accession number(s) (GI #6677833) (NCBI Accession #NP_033138.1) (Uniprot Accession #P08207). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-S100A10 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of S100A10 in C6, 3T3 and Hela cell lysates using S100A10 mouse mAb (1:1000 diluted).Predicted band size:11KDa.Observed band size:11KDa.)
Western Blot (WB) S100A10.

Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-S100A10 mouse mAb (dilution 1:200).)
Immunocytochemistry (ICC) S100A10.

Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using S100A10 mouse mAb.)
Immunoprecipitation (IP) S100A10.

Entrez Summary: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.
UniPort Summary Function: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

Immunogen: Purified recombinant human S100A10 protein fragments expressed in E Coli.
Sensitivity: This antibody detects endogenous levels of S100A10 and does not cross-react with other S100 family proteins.
Sequence Similarities: Belongs to the S-100 family. Subunit Structure: Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with SCN10A.
Subcellular Location: extracellular exosome; extrinsic component of plasma membrane; membrane raft. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing S100A10 are readily searchable from our website. Different antibodies against the same target such as S100A10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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