anti-S1PR1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-S1PR1 antibody; S1PR1; SPHINGOSINE 1- PHOSPHATE RECEPTOR 1;
The S1PR1 s1pr1 (Catalog #MBS219926) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s SPHINGOSINE 1- PHOSPHATE RECEPTOR 1 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB).Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Maximum Dilution: 1/10
Immunofluorescence: Maximum Dilution: 1/500
Immunoprecipitation: Maximum Dilution: 1/300
Western Blotting: Maximum Dilution: 1/5000. Researchers should empirically determine the suitability of the S1PR1 s1pr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The S1PR1 s1pr1 product has the following accession number(s) (GI #13027636) (NCBI Accession #NP_001391.2) (Uniprot Accession #P21453). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Mouse anti Human Sphingosine 1-Phosphate Receptor 1, clone 2B9 recognizes S1P1, otherwise known as CD363/EDG-1, a bioactive lysophospholipid and inducible member of the G-protein coupled receptor superfamily (GPCR) which plays an important role in a variety of cellular processes. During inflammatory diseases such as atherosclerosis the phenotype switching of activated macrophages is influenced by the presence of particular cytokines within the microenvironment. These cells have been characterized as classically activated M1 macrophages, in response to pro-inflammatory molecules such as interferon gamma and lipopolysaccharide, or as alternatively activated M2 macrophages, in response to anti-inflammatory cytokines such as interleukin-4. Activation of S1P1 through its ligand S1P (Sphingosine 1-Phosphate) has been identified as an important promoter of anti-inflammatory M2 macrophages (Hughes et al. 2008). Apoptotic cells can also influence the pro-versus anti-inflammatory macrophage response, and S1P acts as a positive regulator for anti-apoptotic/pro-survival responses by binding to S1P1. Growing evidence suggests that the expression of S1P1 on macrophages may convey protection against apoptosis, at least in part, having implications for the pathogenesis of inflammatory diseases and cancer. Deviation in sphingosine 1-phosphate (S1P) signaling is important in many types of cancer. Analysis of S1P1 and SK1 receptors during the development of tamoxifen resistance in Estrogen Receptor (ER) breast cancer patients concurs that the expression levels of S1P1 correlates with tumor size and Progesterone Receptor (PR) status and that high S1P1 membrane expression is associated with shorter time to recurrence.
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein A. Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing S1PR1 are readily searchable from our website. Different antibodies against the same target such as S1PR1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.