anti-Scn10a antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-SCN10A antibody; SCN10A; Nav1.8;
The Scn10a scn10a (Catalog #MBS802227) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nav1.8 Antibody: FITC reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Nav1.8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).1-10ug/mL (WB), 0.1-1.0ug/mL (Perox) (IHC/ICC), 1.0-10ug/mL (IF). Researchers should empirically determine the suitability of the Scn10a scn10a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Scn10a scn10a product has the following accession number(s) (GI #8394242) (NCBI Accession #NP_058943.1) (Uniprot Accession #Q62968). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Nav1.8 Antibody: FITC with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20 degree C. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic staining and some dull nuclear staining.)
Western Blot (WB) (Western Blot analysis of Monkey COS transient cell lysate showing detection of Nav1.8 protein using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Nav1.8 Monoclonal Antibody, Clone S134-12. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Nav1.8 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Heavy filaggrin-like staining, lower epidermal cells have some staining.)
Background Info: Detects ~220kDa. No cross reactivity against other Nav channels
Scientific Background: Nav1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).