|The SERPINH1 serpinh1 (Catalog #MBS801249) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp47 Antibody: FITC reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Versican can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 (WB). Researchers should empirically determine the suitability of the SERPINH1 serpinh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SERPINH1 serpinh1 product has the following accession number(s) (GI #333360851) (NCBI Accession #NP_001193943) (Uniprot Accession #Q62059). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Hsp47 Antibody: FITC with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Human Epithelial cell (A431) lysates showing detection of ~47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6. Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~47 kDa.)
Background Info: Detects >350kDa
Scientific Background: Hsp47 is a chaperone protein, member of the superfamily of serine proteinase inhibitors. Also known as SERPINH1, a serine proteinase inhibitor. It is a stress protein that resides in the endoplasmic reticulum, has an active role on the intracellular process of folding, assembly and secretion of pro-collagens. Recent studies have shown the association of on an increased expression of Hsp47 around fibrotic lesions (1). The identification of a novel biomarker on cell therapies aimed to reduce the progression of fibrotic diseases, could be used potentially as an universal marker, since Hsp47 binds a single substrate (2). Type I collagen is fundamental during the healing process after a myocardial infarction. It is critical in the position of collagen-produced cells and the assembly of collagen fibrils (3).
Conjugate: FITC. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SERPINH1 are readily searchable from our website. Different antibodies against the same target such as SERPINH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.