anti-SGK1 antibody product blog
Tags: Antibody; Monoclonal Antibody; SGK1; anti-SGK1 antibody; SGK;
The SGK1 sgk1 (Catalog #MBS6000372) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SGK, phosphorylated (Ser78) (Serine/threonine-protein Kinase Sgk1, Serum/glucocorticoid Regulated Kinase 1, SGK1) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SGK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,
0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the SGK1 sgk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SGK1 sgk1 product has the following accession number(s) (GI #219521878) (NCBI Accession #NP_001137149.1) (Uniprot Accession #O00141). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SGK, phosphorylated (Ser78) (Serine/threonine-protein Kinase Sgk1, Serum/glucocorticoid Regulated Kinase 1, SGK1) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from COS cells transfected with SGK1 using Phospho-SGK1 (Ser78) Rabbit mAb #MBS6000372, untreated or following antibody pre-incubation with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide that cannot block the signal.)
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, untreated (-) or treated with H2O2 (4mM for 15 min), using Phospho-SGK1 (Ser78) #MBS6000372 (upper) or SGK1 Antibody MBS618338 (lower).)
Western Blot (WB) (Western blot analysis of extracts from COS cells, untransfected (-) or transfected with human SGK1 (+), using Phospho-SGK1 (Ser78) #MBS6000372 (upper) or SGK1 Antibody #MBS618338 (lower).)
Serum and glucocorticoid-inducible kinase (SGK) is a serine/threonine kinase closely related to Akt. SGK is rapidly induced in response to a variety of stimuli, including serum, lucocorticoid, follicle stimulating hormone, osmotic shock, and mineralocorticoids. SGK activation can be accomplished via HGF PI3K-dependent pathways and by integrin-mediated PI3K-independent pathways. Induction and activation of SGK has been implicated in activating the modulation of antiapoptotic and cell cycle regulation. SGK also plays an important role in activating certain potassium, sodium, and chloride channels, suggesting its involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK is negatively regulated by ubiquitination and proteasome degradation. The MAP kinase family member BMK1 interacts with and activates SGK by phosphorylation at serine 78.