anti-SIGLEC-5/SIGLEC-14 antibody product blog
Tags: Antibody; Monoclonal Antibody; SIGLEC-5/SIGLEC-14; anti-SIGLEC-5/SIGLEC-14 antibody;
The SIGLEC-5/SIGLEC-14 siglec5 (Catalog #MBS224673) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s SIGLEC-5/SIGLEC-14 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/10. Researchers should empirically determine the suitability of the SIGLEC-5/SIGLEC-14 siglec5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SIGLEC-5/SIGLEC-14 siglec5 product has the following accession number(s) (GI #4502659) (NCBI Accession #NP_003821.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN SIGLEC-5/SIGLEC-14:FITC with the following immunoassay(s):
Testing Data #1 (Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14 (MBS224725) followed by rabbit F(ab\')2 anti mouse IgG:FITC (MBS235160))
Testing Data #2 (Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14:FITC (MBS224673) )
Testing Data #3 (Human peripheral blood monocytes stained with Mouse anti Human Siglec-5/Siglec-14:RPE (MBS224544) )
MBS224725 recognises human Siglec-5 (Sialic acid-binding Ig-like lectin 5), otherwise known as CD170, a novel sialic-acid-binding Ig-like lectin, and member of the Ig superfamily, expressed by dendritic cells (DCs), activated macrophages, neutrophils, and cells of the monocyte/myeloid lineage. Clone 1A5, is one of several Siglec-5 antibodies which also recognises human Siglec-14 (Sialic acid-binding Ig-like lectin 14), see Angata, T. et al. for details. Siglec-14 shares an almost identical sequence with Siglec-5 within the first two Ig-like domains, indicating partial gene conversion between these two Siglecs, also evident in other primate species. Siglec-5 is highly related to the myelomonocytic-derived adhesion molecule CD33 (Siglec-3), and mediates sialic-acid dependent binding to cells, as well as acting as an inhibitory receptor in the down-regulation of cell activation. Structurally, Siglec-5 contains an immunoreceptor tyrosine-based inhibitor motif (ITIM), which plays a part in the modulation of cellular responses, and when phosphorylated, can bind to the SH2 domain of several SH2-containing phosphatases. Siglec-14 is a putative sialic-acid binding adhesion molecule, and member of the Ig superfamily, predominantly expressed in hematopoietic tissues, which has been shown to associate with the activating adapter protein DAP12. Clone 1A5 has been show to cross react with Chimpanzee, see Jaroenpool, J. et al. for details.
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Siglec-5-Fc protein, consisting of the full-length extracellular region of human Siglec-5, fused with the Fc region of human IgG1.
Buffer Solution: Phosphate buffered saline
Target Species: Human.