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anti-SIGLEC-7 antibody product blog

Posted on 2015-11-26 09:37:55 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; Siglec-7; anti-Siglec-7 antibody;
The SIGLEC-7 siglec7 (Catalog #MBS224402) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s SIGLEC-7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells.
Western Blot: This item detects a band of approximately 65kDa using Siglec-7 transfected CHO cells see Nicoll, G. et al. for details.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the SIGLEC-7 siglec7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The SIGLEC-7 siglec7 product has the following accession number(s) (GI #469469047) (NCBI Accession #NP_001264130.1) (Uniprot Accession #Q9Y286). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN SIGLEC-7 with the following immunoassay(s):
Testing Data #1 (Human peripheral blood lymphocytes stained with Mouse anti Human Siglec-7: Endotoxin Low (MBS224600))
Testing Data #1 SIGLEC-7.

Testing Data #2 (Human peripheral blood lymphocytes stained with Mouse anti Human Siglec-7: FITC (MBS224698))
Testing Data #2 SIGLEC-7.

Testing Data #3 (Human peripheral blood lymphocytes stained with Mouse anti Human Siglec-7: RPE)
Testing Data #3 SIGLEC-7.

Mouse anti Human Siglec-7 antibody, cloneS7.7 recognizes human Sialic acid-binding Ig-like lectin 7, otherwise known as CD328, a putative adhesion molecule and member of the Ig superfamily, predominantly expressed by both resting and activated natural killer (NK) cells, and also at lower levels by monocytes, granulocytes, monocyte-derived macrophages and dendritic cells, and a small population of CD8+ memory T cells. Classed as a CD33-related Siglec, Siglec-7 preferentially binds to glycoconjugates containing alpha-2,3- or alpha-2,6-linked sialic acid, and acts as a novel inhibitory receptor for NK cells, mediating the inhibition of NK cell cytotoxicity, and implicated in the regulation of NK cell and T cell activation, and hematopoiesis. Mouse anti Human Siglec-7 antibody, cloneS7.7 is a blocking antibody (Avril et al. 2006), and has been shown to recognize Siglec-7 in Rhesus monkey (Jaroenpool et al. 2007).

Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Siglec-7 3T3 transfected cells
Fusion Partners: Spleen cells from immunised Balb/c mice, were fused with cells of the Sp2/0 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SIGLEC-7 are readily searchable from our website. Different antibodies against the same target such as SIGLEC-7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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