anti-Siglech antibody product blog
Tags: Antibody; Monoclonal Antibody; Siglec-H; anti-Siglech antibody; Siglech;
The Siglech n/a (Catalog #MBS6000962) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Siglec-H (Siglech, Sialic Acid Binding Ig-like Lectin H, 6430529G09Rik, MGC106898) (Biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Siglec-H can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry and Immunohistochemistry.
Dilution: Flow Cytometry: 1:50
Immunohistochemistry: Frozen. Researchers should empirically determine the suitability of the Siglech n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Siglech n/a product has the following accession number(s) (GI #188497640) (NCBI Accession #NP_848821.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Siglec-H is a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. This monoclonal antibody exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen. Incubation of IPC with this antibody in vitro reduces secretion of IFN-alpha in response to CpG DNA without causing IPC depletion.
Immunogen: Purified bone-marrow derived mouse IPC. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Siglech are readily searchable from our website. Different antibodies against the same target such as Siglech may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.