anti-SIRT1 antibody product blog
Tags: Antibody; Monoclonal Antibody; SirT1; anti-SIRT1 antibody;
The SIRT1 sirt1 (Catalog #MBS475614) is an Antibody produced from MouseSource: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT1 Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SIRT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
WB: 1:500
ICC: 1:100. Researchers should empirically determine the suitability of the SIRT1 sirt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SIRT1 sirt1 product has the following accession number(s) (GI #215982798) (NCBI Accession #NP_001135970.1) (Uniprot Accession #Q96EB6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-SIRT1 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of SIRT1 in A549, 293, Jurkat and Hela cell lysates using SIRT1 mouse mAb (dilution 1:500).Predicted band size:120KDa.Observed band size:120KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry of HeLa cells using SIRT1 mouse mAb (dilution 1:100).)
Entrez Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008].
Immunogen: Purified recombinant fragment of human SIRT1 expressed in E Coli.
Sensitivity: The antibody detects endogenous level of total SIRT1 protein.
Sequence Similarities: Belongs to the sirtuin family. Class I subfamily. Contains 1 deacetylase sirtuin-type domain. Subunit Structure: Found in a complex with PCAF and MYOD1. Interacts with FOXO1; the interaction deacetylates FOXO1, resulting in its nuclear retention and promotion of its transcriptional activity Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with HES1, HEY2 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus); the interaction disrupts the interaction between SIRT1 and p53/TP53. Interacts with SETD7; the interaction induces the dissociation of SIRT1 from p53/TP53 and increases p53/TP53 activity. Interacts with MYCN, NR1I2, CREBZF, TSC2, TLE1, FOS, JUN, NR0B2, PPARG, NCOR, IRS1, IRS2 and NMNAT1. Interacts with HNF1A; the interaction occurs under nutrient restriction. Interacts with SUZ12; the interaction mediates the association with the PRC4 histone methylation complex which is specific as an association with PCR2 and PCR3 complex variants is not found. Interacts with HIV-1 tat.