|The Slc17a7 slc17a7 (Catalog #MBS802712) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VGLUT1 Antibody: ATTO 700 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s VGLUT1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB).
1:1000 (WB). Researchers should empirically determine the suitability of the Slc17a7 slc17a7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Slc17a7 slc17a7 product has the following accession number(s) (GI #16758726) (NCBI Accession #NP_446311.1) (Uniprot Accession #Q62634). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VGLUT1 Antibody: ATTO 700 with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Tissue: spinal cord. Species: Mouse. Fixation: 4% PFA. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:500 for 16 hours at RT. Secondary Antibody: Alexa Fluor 555 Donkey Anti-Mouse (red) at 1:2000 for 2 hours at RT. Counterstain: NeuN neuronal stain (green). Magnification: 20X. Courtesy of: Leilei Wang, Ph.D. UT Southwestern Medical Center at Dallas.)
Western Blot (WB) (Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody at 1:1000.)
Background Info: Detects ~52kDa. No cross-reactivity against VGLUT2.
Scientific Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).
Conjugate: ATTO 700. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Slc17a7 are readily searchable from our website. Different antibodies against the same target such as Slc17a7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.