|The SMAD3 smad3 (Catalog #MBS246413) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 7F3] (IgG1,k) to Human SMAD3 reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SMAD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA, IHC-P (2.5 ug/ml), WB
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western blot using tissue lysates, cell lysates and the recombinant protein used as the immunogen. Researchers should empirically determine the suitability of the SMAD3 smad3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMAD3 smad3 product has the following accession number(s) (GI #5174513) (NCBI Accession #NP_005893.1) (Uniprot Accession #P84022). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 7F3] (IgG1,k) to Human SMAD3 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-SMAD3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
Western Blot (WB) (SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in HeLa.)
Western Blot (WB) (SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in Jurkat.)
Western Blot (WB) (SMAD3 monoclonal antibody clone 7F3. Western blot of SMAD3 expression in human colon.)
ELISA (EIA) (Detection limit for recombinant GST tagged SMAD3 is approximately 0.1 ng/ml as a capture antibody.)
SMAD3, a transcriptional regulator, is activated by TGF-beta and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.
Target Species: Human
Immunogen Description: SMAD3 (NP_005893, 120 a.a. ~ 222 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen: SMAD3 antibody was raised against sMAD3 (NP_005893, 120 a.a. ~ 222 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMAD3 are readily searchable from our website. Different antibodies against the same target such as SMAD3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.