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anti-SMAD5 antibody product blog

Posted on 2015-10-11 19:38:15 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-SMAD5 antibody; SMAD5;
The SMAD5 smad5 (Catalog #MBS850290) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMAD5 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SMAD5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA).
WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the SMAD5 smad5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The SMAD5 smad5 product has the following accession number(s) (GI #47778929) (NCBI Accession #NP_001001419.1) (Uniprot Accession #Q99717). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMAD5 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.)
Western Blot (WB) SMAD5.

Immunohistochemistry (IHC) (Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.)
Immunohistochemistry (IHC) SMAD5.

Immunofluorescence (IF) (Figure 3: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF) SMAD5.

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Immunogen: Purified recombinant fragment of human SMAD5 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMAD5 are readily searchable from our website. Different antibodies against the same target such as SMAD5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. SMAD5 also interacts with the following gene(s): ACVR1, BMP4, BMP6, BMP7, BMPR1A, PPM1A, SKI, SMAD4, SMURF1, SMURF2. Blood, Bone, Connective Tissue, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Vascular tissues are correlated with this protein.
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