anti-SMARCA4 antibody product blog
Tags: Antibody; Monoclonal Antibody; SMARCA4; anti-SMARCA4 antibody; BRG1;
The BRG1 smarca4 (Catalog #MBS475232) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-BRG1 Mouse mAb reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s BRG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP).WB: 1:1000
ICC: 1:50. Researchers should empirically determine the suitability of the BRG1 smarca4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The BRG1 smarca4 product has the following accession number(s) (GI #192807312) (NCBI Accession #NP_001122316.1) (Uniprot Accession #P51532). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Belongs to the SNF2/RAD54 helicase family. Contains 1 bromo domain. Contains 1 helicase ATP-Bindin g domain. Contains 1 helicase C-terminal domain. Contains 1 HSA domain. Contains 1 QLQ domain.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-BRG1 Mouse mAb with the following immunoassay(s):
Western Blot (WB) (Western blot detection of BRG1 in Hela NE, F9 and K562 cell lysates using BRG1 mouse mAb (1:1000 diluted).Predicted band size:220KDa.Observed band size:220KDa.)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells fixed with 4% Paraformaldehyde and using anti-BRG1 mouse mAb (dilution 1:50). DAPI was used to stain nucleus (blue).)
Immunoprecipitation (IP) (Immunoprecipitation analysis of K562 cell lysates using BRG1 mouse mAb (MBS475232).)
Swiss-Prot Acc.P51532.ATP-dependent chromatin remodeling complexes play an essential role in theregulation of various nuclear processes, such as gene expression, DNA replication, and repair. Acts asa corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
Modification: Unmodification
Source: Human
Antibody type: Primary Antibody
Conjugation: Unconjugated
Immunogen: Purified recombinant human BRG1 protein fragments expressed in E Coli.
Sequence Similarities: Belongs to the SNF2/RAD54 helicase family. Contains 1 bromo domain. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 1 HSA domain. Contains 1 QLQ domain. Subcellular Location: Nucleus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing BRG1 are readily searchable from our website. Different antibodies against the same target such as BRG1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.