anti-SMC1 antibody product blog
Tags: Antibody; Monoclonal Antibody; SMC1; anti-SMC1 antibody;
The SMC1 smc1a (Catalog #MBS850029) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SMC1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SMC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA).WB: 1:500-1:2000
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA: 1:10000. Researchers should empirically determine the suitability of the SMC1 smc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMC1 smc1a product has the following accession number(s) (GI #30581135) (NCBI Accession #NP_006297.2) (Uniprot Accession #Q14683). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMC1 Antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1. Western blot analysis using SMC1 mouse mAb against K562 (1), Jurkat (2) and A549 (3) cell lysate)
Immunohistochemistry (IHC) (Figure 2. Immunohistochemical analysis of paraffin-embedded human colon using SMC1 mouse mAb with DAB staining.)
Flow Cytometry (FC/FACS) (Figure 3. Flow cytometric analysis of Hela cells using SMC1 mouse mAb (green) and negative control (purple).)
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Immunogen: Purified recombinant fragment of human SMC1 expressed in E. Coli. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMC1 are readily searchable from our website. Different antibodies against the same target such as SMC1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.