anti-SOAT1 antibody product blog
Tags: Antibody; Monoclonal Antibody; SOAT1; STAT 1; anti-SOAT1 antibody;
The SOAT1 soat1 (Catalog #MBS633102) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STAT 1, phosphorylated (Tyr701) (Signal Transducer and Activator of Transcription 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s STAT 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1-2ug/ml
ELISA: 0.1-1ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded sections): 5ug/ml; Heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required. Researchers should empirically determine the suitability of the SOAT1 soat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SOAT1 soat1 product has the following accession number(s) (GI #49533617) (NCBI Accession #NP_003092.4) (Uniprot Accession #P35610). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors. In response to treatment of target cells with cytokines or in some cases growth factors, STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding which results in transcriptional activation of distinct target genes. Phosphorylation of a conserved tyrosine residue located near the carboxy-terminus of all STAT proteins is required for both dimerization and DNA binding. Tyrosine phosphorylation is therefore an useful marker for STAT activation. At least one and oftentimes several STAT proteins are activated in response to cytokines that utilize receptors from the cytokine receptor superfamily. Nevertheless, a striking specificity of specific STAT activation is seen in response to individual cytokines. Stimulation of responsive cells with IFNa/b induces the formation of a transcription complex termed ISGF3. This complex binds to the interferon-stimulated response element (ISRE), activating the transcription of responsive genes. The ISGF3 complex consists of tyrosine phosphorylated STAT1a, STAT1b (p84), STAT2 and p48, a 48kD DNA binding protein that is specific for the IFN-stimulated response element. Formation of this complex and its migration into the nucleus is dependent upon tyrosine phosphorylation of STAT1a/b and STAT2. Stimulation of cells with IFN-g results in tyrosine phosphorylation of STAT1a (p91), but not of STAT2. Phosphorylation of STAT1a results in its homodimerization and migration into the nucleus where it binds to the IFN-g activated site (GAS). The STAT1a and STAT1b isoforms arise by alternative splicing of a single gene. The only difference between the two proteins is that STAT1b lacks 38 carboxy-terminal amino acids. Contained within these 38 terminal amino acids of STAT1a is a critical serine residue (Ser- 727) whose phosphorylation is required for maximal IFN-g induced transcription.