anti-ssr4 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-SSR4 antibody; SSR4;
The ssr4 ssr4 (Catalog #MBS6009598) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SSR4 (TRAPD, Translocon-associated Protein Subunit delta, Signal Sequence Receptor Subunit delta) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SSR4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunofluorescence (IF).Suitable for use in ELISA, Western Blot and Immunofluorescence.
Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the ssr4 ssr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ssr4 ssr4 product has the following accession number(s) (GI #19112609) (NCBI Accession #NP_595817.1) (Uniprot Accession #Q9P7Y0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
EACLEPQITP SYYTTSDAVI STETVLIVEI SLTCKNRVQN MALYADVGGK QFPVTRGQDV GRYQVSWSLD HKSAHAGTYE VRFFDEESYS LLRKAQRNNE DISIIPPLFT VSVDHRGTWN GPWVSTEVLA AAIGLVIYYL AFSAKSHIQA *.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SSR4 (TRAPD, Translocon-associated Protein Subunit delta, Signal Sequence Receptor Subunit delta) with the following immunoassay(s):
Western Blot (WB) (Western Blot detection against Immunogen (42.61kD).)
Western Blot (WB) (SSR4 monoclonal antibody, Western Blot analysis of SSR4 expression in C32.)
Western Blot (WB) (Western Blot analysis of SSR4 expression in transfected 293T cell line by SSR4 monoclonal antibody.|Lane 1: SSR4 transfected lysate (19kD).|Lane 2: Non-transfected lysate.)
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to SSR4 on HeLa cell. [antibody concentration 10ug/ml].)
Testing Data (Detection limit for recombinant GST tagged SSR4 is ~0.1ng/ml as a capture antibody.)
SSR4, also called TRAPD, is assumed to be involved in protein secretion. It is located in the Xq28 region, arranged in a compact head-to-head manner with the IDH3G gene. These two genes are driven by a bidirectional promoter located between them, and encode proteins involved in unrelated biochemical pathways located in different compartments of the cell. The nontranscribed intergenic region represents only 133bp and is embedded in a CpG island. The CpG island functions as a bidirectional promoter to initiate the transcription of both functionally unrelated genes with distinct expression patterns. SSR4 consists of six exons and is approximately 70kb telomeric to the ALD gene. Although alternative splicing of exon 5 has not been detected in human SSR4, transcript variants missing the region homologous to human exon 5 have been detected in both Xenopus laevis and Mus musculus.
Immunogen: Full length recombinant corresponding to aa24-174 from human SSR4 (AAH03371) with GST tag. MW of the GST tag alone is 26kD.