anti-Stat92E antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-Stat92E antibody; Stat92E; STAT 5;
The Stat92E stat92e (Catalog #MBS631781) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STAT 5, phosphorylated (Signal Transducer and Activator of Transcription 5) reacts with Bovine, Human, Rat, Sheep and may cross-react with other species as described in the data sheet. MyBioSource\'s STAT 5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in Western Blot and ELISA.
Dilution: Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml. Researchers should empirically determine the suitability of the Stat92E stat92e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Stat92E stat92e product has the following accession number(s) (GI #442620068) (NCBI Accession #NP_001262762.1) (Uniprot Accession #Q24151). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
STATs (signal transducers and activators of transcription) were originally identified as DNA-binding proteins essential to interferon IFNa- and IFNg-regulated gene expression. Since then, several additional mammalian STATs, a Drosophila STAT (D-STAT or Marelle), and a STAT-like protein from Dictyostelium discoideum have been recognized. In unstimulated cells STAT proteins exist largely as latent cytoplasmic transcription factors. Treatment of cells with cytokines or certain growth factors results in the tyrosine phosphorylation (via Janus kinases for cytokines) of specific STATs causing them to form homodimers or heterodimers that enter the nucleus. In the nucleus, STAT dimers bind DNA and activate transcription of distinct target genes. Although several different STATs may be activated in response to a given ligand, there is often a striking specificity in the pattern of STAT activation. Mammalian STAT proteins share several conserved structural and functional domains as illustrated below: STAT5 (mammary growth factor) was originally isolated as a tyrosine phosphorylated DNA binding protein from prolactin stimulated mammary tissue and IL-3 stimulated myeloid cells. Subsequently, two highly homologous (~96%) genes, Stat5a and Stat5b, were identified in mouse.7-8 Both STATs are expressed in most tissues of virgin and lactating mice although differential accumulation of STAT5a and STAT5b mRNAs occurs in muscle and mammary tissues. STAT5 activation has been observed in response to IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GM-CSF, TPO, EPO, GH, and prolactin (PRL). STAT5a and STAT5b proteins recognize the GAS (gamma interferon activating sequence) site TTCNNNGAA with the preferred DNA-binding consensus site deduced as TTCC(A>T)GGAA. Western blot analysis of the full-length STAT5a protein, isolated from a variety of cultured cells, indicates that the protein migrates with a molecular weight of ~95kD. C-terminal truncated forms of the STAT5a protein have also been reported.