anti-STUB1 antibody product blog
Tags: Antibody; Monoclonal Antibody; STUB1; anti-STUB1 antibody;
The STUB1 stub1 (Catalog #MBS9230982) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STUB1 Antibody (C-term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s STUB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).WB: 1:4000. Researchers should empirically determine the suitability of the STUB1 stub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The STUB1 stub1 product has the following accession number(s) (GI #645912990) (NCBI Accession #NP_001280126.1) (Uniprot Accession #Q9UNE7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the STUB1 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-STUB1 Antibody (C-term) at 1:4000 dilution
Lane 1: 293 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: L929 whole cell lysate
Lysates/proteins at 20 ug per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 35 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Collaborates with ATXN3 in the degradation of misfolded chaperone substrates: ATXN3 restricting the length of ubiquitin chain attached to STUB1/CHIP substrates and preventing further chain extension. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at \'Lys-41\', \'Lys-61\' and \'Lys-81\', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. Ubiquitinates EPHA2 and may regulate the receptor stability and activity through proteasomal degradation. Negatively regulates the suppressive function of regulatory T-cells (Treg) during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner (PubMed:23973223).
Antigen Type: Recombinant Protein
Antigen Source: Human. Cellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to inflammatory signals in regulatory T-cells (Treg)
Tissue Location: Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing STUB1 are readily searchable from our website. Different antibodies against the same target such as STUB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.