|The STX1A stx1a (Catalog #MBS801122) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Syntaxin Antibody: ATTO 390 reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Syntaxin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).
1:1000 (WB). Researchers should empirically determine the suitability of the STX1A stx1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The STX1A stx1a product has the following accession number(s) (GI #4759182) (NCBI Accession #NP_004594.1) (Uniprot Accession #Q16623). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Syntaxin Antibody: ATTO 390 with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Syntaxin Monoclonal Antibody, Clone SP-6. Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Syntaxin Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle positive, basal layer staining in the epidermis and positive staining in the hair follicles.)
Western Blot (WB) (Western Blot analysis of Rat tissue lysate showing detection of Syntaxin protein using Mouse Anti-Syntaxin Monoclonal Antibody, Clone SP-6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Syntaxin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects ~38kDa.
Scientific Background: Syntax ins are a family of membrane integrated Q-SNARE proteins participating in exocytosis (1). They possess a single C-terminal transmembrane domain, a SNARE domain, and an N-terminal regulatory domain. This SNARE domain binds to synaptobrevin and SNAP-25 to form the SNARE complex (2, 3). Support of syntaxin\'s involvement in vesicular transport and neurotransmission includes the requirement of its yeast homologs, SSO 1 and 2, in targeting and fusion of Golgi-derived vesicles with the plasma membrane (4). Syntaxin also provides the target pf clostridial neurotoxin C1, which blocks neurotransmitter release (5). The formation of 20S complex involves the interaction of VAMP with syntaxin through an ATP-dependent reaction modulated by synaptotagmin, SNAP-25, a-SNAP and NSF (6).
Conjugate: ATTO 390. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing STX1A are readily searchable from our website. Different antibodies against the same target such as STX1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.