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anti-Syt10 antibody product blog

Posted on 2016-01-27 00:43:26 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; Synaptotagmin 10; anti-Syt10 antibody; Syt10;
The Syt10 syt10 (Catalog #MBS802885) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Synaptotagmin-10 Antibody: ATTO 680 reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Synaptotagmin 10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC).
1:1000 WB. Researchers should empirically determine the suitability of the Syt10 syt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Syt10 syt10 product has the following accession number(s) (GI #9055358) (NCBI Accession #NP_061273.1) (Uniprot Accession #Q9R0N4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Synaptotagmin-10 Antibody: ATTO 680 with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat brain lysates showing detection of Synaptotagmin 10 protein using Mouse Anti-Synaptotagmin 10 Monoclonal Antibody, Clone S269-73. Primary Antibody: Mouse Anti-Synaptotagmin 10 Monoclonal Antibody at 1:100, 1:250, 1:500, and 1:1000.)
Western Blot (WB) Syt10.

Background Info: Detects ~60kDa. Does not cross-react with Synaptotagmin-6 or Synaptotagmin-3 (or others).

Scientific Background: Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.

Conjugate: ATTO 680. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Syt10 are readily searchable from our website. Different antibodies against the same target such as Syt10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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