anti-TCR V beta 3 antibody product blog
Tags: Antibody; Monoclonal Antibody; TCR V beta 3; anti-TCR V beta 3 antibody;
The TCR V beta 3 n/a (Catalog #MBS666251) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human TCR V beta 3-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TCR V beta 3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the TCR V beta 3 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human TCR V beta 3-Purified Preservative Free with the following immunoassay(s):
Testing Data
Information: Human T cell receptor (TCR) is a T cell surface expressed heterodimer of alpha/beta or gamma/delta chains. TCR\'s recognize antigen peptides bound to MHC molecules forming the basis of antigen specific response by T cells. Antibody JOVI-3 is specific for the human Vb3 TCR gene product and reacts with 1-10% of T cells in normal human blood. Antibody JOVI-3 is mitogenic for T cells expressing TCR Vb3.
Performance: Five x 105 cultured CH7C17 tumor cells (HA1.7 Vb3+ transfectant) were washed and incubated 45 minutes on ice with 80 ul of anti-TCR Vb3 at 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.9 log10 fluorescent units when compared to a Mouse IgG2a isotype control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen: Human HA1.7 TCR beta chain expressed on transgenic mouse cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TCR V beta 3 are readily searchable from our website. Different antibodies against the same target such as TCR V beta 3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.