anti-TGF beta R2 antibody product blog
Tags: Antibody; Monoclonal Antibody; TGF beta R2; anti-TGF beta R2 antibody;
The TGF beta R2 tgfbr2 (Catalog #MBS690878) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse TGF beta R2 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s TGF beta R2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).1. Western blotting: Yes (1:500-1000). Researchers should empirically determine the suitability of the TGF beta R2 tgfbr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TGF beta R2 tgfbr2 product has the following accession number(s) (GI #239787881) (NCBI Accession #NP_033397.3) (Uniprot Accession #Q62312). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Most cell types express three sizes of receptors for TGF-beta. These are designated Type I (53 kDa), Type II (70 - 85 kDa), and Type III (250 - 350 kDa). The Type I receptor is a membrane-bound serine/threonine kinase that apparently requires the presence of the Type II receptor to bind TGF-beta. The Type II receptor is also a membrane-bound serine/threonine kinase that binds TGF-beta1 and TGF-beta 3 with high affinity and TGF-beta 2 with much lower affinity. The Type I and Type II receptors together form a heterodimeric signaling complex that is essential for the transduction of the anti-proliferative signals of TGF-beta. The Type III receptor is a transmembrane proteoglycan with a large extracellular domain and a 43 amino acid residue cytoplasmic domain. The cytoplasmic domain of the Type III receptor lacks an obvious signaling motif and the receptor may not be involved directly in signal transduction.
Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TGF- R2 extracellular doamin. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TGF beta R2 are readily searchable from our website. Different antibodies against the same target such as TGF beta R2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.