anti-THY1 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD90; anti-THY1 antibody; THY1;
The CD90 cd90 (Catalog #MBS8510531) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human CD90, Purified mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD90 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CD90 cd90 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD90 cd90 product has the following accession number(s) (GI #224697007) (NCBI Accession #CAM58077.1) (Uniprot Accession #B9ZSM8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse anti-Human CD90, Purified mAb with the following immunoassay(s):
Flow Cytometry (FC/FACS) (HEL Cells with Purified CD90 mAb 4AeBio5E10AD2, followed by anti-mouse IgGs-PE)
The 4AeBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34-cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.
Experimental Methods: 1.Take 100mul 10e6 HEL Cells and add to the bottom of 5ml tube;
2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
4.Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
6.Repeat step 5.
7.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD90 are readily searchable from our website. Different antibodies against the same target such as CD90 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.