anti-TLR3 antibody product blog
Tags: Antibody; Monoclonal Antibody; TLR3; anti-TLR3 antibody;
The TLR3 n/a (Catalog #MBS607172) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLR3 (Toll Like Receptor 3) reacts with Canine, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TLR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry.
Dilution: Flow Cytometry: 2-4ug/1x10e6 Ramos cells for intracellular staining
Western Blot: 1-3ug/ml
Immunoprecipitation: 2ug/10e6 transfected cells
Immunohistochemistry: Paraffin sections. Researchers should empirically determine the suitability of the TLR3 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TLR3 n/a product has the following accession number(s) (GI #237871857). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TLR3 (Toll Like Receptor 3) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MBS607172 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 ug/lane human intestine tissue lysate (lane C).)
Flow Cytometry (FC/FACS) (Flow cytometric analysis of TLR3 on Ramos cells using MBS607172 at 1 ug/ 1X10^6 cells.)
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120kD (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.
Immunogen: Synthetic peptide corresponding to aa 55-70 [VLNLTHNQLRRLPAAN] of human TLR3. Positive Control: Human intestine or Ramos cells (for intracellular staining). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TLR3 are readily searchable from our website. Different antibodies against the same target such as TLR3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.