anti-TLR9 antibody product blog
Tags: Antibody; Monoclonal Antibody; TLR9; anti-TLR9 antibody;
The TLR9 tlr9 (Catalog #MBS584246) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLR9, Mouse, mAb 5G5 reacts with Reactivity: Mouse. Cross reactivity: Canine, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TLR9 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), Immunoassay (IA), P, Western Blot (WB).Application Notes: The monoclonal antibody 5G5 can be used for flow cytometry, Western blotting and immuno assays as detection antibody. Furthermore, the monoclonal antibody 5G5 is useful for immunohistology on frozen and paraffin sections. The monoclonal antibody 5G5 stains RAW macrophages and TLR9 (CD289) transfected HEK293 cells. The antibody is weakly cross reactive with human TLR9.
Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the TLR9 tlr9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TLR9 tlr9 product has the following accession number(s) (GI #74054321) (NCBI Accession #AAZ95520.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: The monoclonal antibody 5G5 reacts with the Toll-like receptor 9 (TLR9, CD289). TLRs are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila, toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLRs identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence to pathogens. As investigated so far all functional characterized TLR signal via the TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to be essential. In contrast to cell-wall components, bacterial DNA is probably invisible for immune cells until DNA is liberated during processes taking place in the endosomal/lysosomal compartment where intracellular TLR9 recruits MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide-containing sequences are found much more frequently in bacterial genomes than in vertebrates genomes, whereas the frequency of CpG dinucleotides are suppressed and usually methylated. The regions adjacent to the CpG dinucleotides also affect the immunostimulatory activity. The optimal sequence differs significantly between mammalian species. Methylated CpG dinucleotides lack immunostimulatory activities. Cellular activation in response to bacterial DNA and synthetic dinucleotides containing unmethylated CpG-dinucleotides is mediated by TLR9. The monoclonal antibody 5G5 reacts with RAW macrophages and TLR9 transfected HEK293 cells, and it is cross reactive with canine TLR9.