anti-TNF antibody product blog
Tags: Antibody; TNF; Monoclonal Antibody; anti-TNF antibody; TNF-alpha;
The TNF tnf (Catalog #MBS438099) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody reacts with Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish and may cross-react with other species as described in the data sheet. MyBioSource\'s TNF-alpha (Tumor Necrosis Factor alpha) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin/Paraffin.Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Immunohistology (Formalin-paraffin) (2-4ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the TNF tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TNF tnf product has the following accession number(s) (GI #25952111) (NCBI Accession #NP_000585.2) (Uniprot Accession #P01375). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TNF-alpha (Tumor Necrosis Factor alpha) Mouse Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Formalin paraffin-embedded human Erdheim Chester disease (also known as polyostotic sclerosing histiocytosis) stained with TNF alpha Monoclonal Antibody (TNF706).)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNF706))
Immunofluorescence (IF) (Immunofluorescence staining of HePG2 cells using TNF alpha Mouse Monoclonal Antibody followed by goat anti-mouse IgG(green). Nuclei are stained with Reddot.)
Cellular Localization: Cytoplasmic and extracellular (Secreted)
Immunogen: Recombinant N-terminal fragment of human TNF-alpha. Hu-Chromosome Location: 6p21.33
Positive Control: HePG2, HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TNF are readily searchable from our website. Different antibodies against the same target such as TNF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Connective Tissue, Heart, Intestine, Lung, Lymph Node, Ovary, Placenta, Prostate tissues are correlated with this protein.