anti-TNF-RI antibody product blog
Tags: Antibody; Monoclonal Antibody; TNF-RI; anti-TNF-RI antibody;
The TNF-RI tnfrsf1a (Catalog #MBS584088) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The TNF-RI, Mouse, mAb 100 �g 300 reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s TNF-RI can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), Immunoassay (IA), Immunoprecipitation (IP).Application Notes: The monoclonal antibody HM104 can be used for immuno assay, and flow cytometry. Furthermore, the monoclonal antibody HM104 is useful for immuno precipitation and immunohistology on frozen sections. Be aware that the reactivity of the antibody with soluble TNF-Receptor is inhibited by high concentrations of both mouse and human TNF-alpha.
Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the TNF-RI tnfrsf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TNF-RI tnfrsf1a product has the following accession number(s) (GI #2290398) (NCBI Accession #AAB65143.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: The monoclonal antibody HM104 recognizes the extracellular part of the Tumor Necrosis Factor Receptor type I (TNF-RI) of the membrane-bound as well as the soluble receptor. TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TNF-RI are readily searchable from our website. Different antibodies against the same target such as TNF-RI may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.