anti-TOP2A antibody product blog
Tags: Antibody; Monoclonal Antibody; Topoisomerase II alpha; anti-TOP2A antibody; TOP2A;
The TOP2A top2a (Catalog #MBS179499) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Topoisomerase II alpha Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Topoisomerase II alpha can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blot (WB).WB: 1:500-1:2000
IHC: 1:50-1:200
IP: 1:50. Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TOP2A top2a product has the following accession number(s) (GI #19913406) (NCBI Accession #NP_001058.2) (Uniprot Accession #P11388). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Topoisomerase II alpha Rabbit Monoclonal Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human colon cancer, using Topoisomerase II alpha Antibody(MBS179499)
TOP2A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TOP2A Antibody (MBS179499)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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Western Blot (WB) (Western blot analysis of Topoisomerase II alpha expression in (1) HeLa cell lysate; (2) Jurkat cell lysate (MBS179499).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP2A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TOP2A )
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes. May play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation (By similarity).