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anti-Trak2 antibody product blog

Posted on 2016-04-24 12:46:28 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; anti-TRAK2 antibody; TRAK2;
The Trak2 trak2 (Catalog #MBS807375) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human Trak2 Monoclonal IgG2A reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Trak2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF).
Western Blot: 1:1000. Researchers should empirically determine the suitability of the Trak2 trak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Trak2 trak2 product has the following accession number(s) (GI #188497667) (NCBI Accession #NP_055864.2) (Uniprot Accession #O60296). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Anti-Human Trak2 Monoclonal IgG2A with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Rat Brain-Membrane showing detection of ~ 100 kDa TRAK2 protein using Mouse Anti-TRAK2 Monoclonal Antibody, Clone S390-43. Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-TRAK2 Monoclonal Antibody at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 100 kDa.)
Western Blot (WB) Trak2.

TRAK2, a member of the TRAK family of adaptor proteins, links mitochondria to microtubule based motors. Localized in dendrites, it is specifically required for dendritic development as it reacts with dynein and dynactin. TRAK2 interferes with kinesin-1 binding and axonal transport (1). It has been shown to interact with Kir2.1 and GABA-R Beta2 (2, 3).

Immunogen: Fusion protein amino acids 757-914 (C-terminus) of human TRAK2
Immunogen Species: Human. COA: A 1:100 dilution was sufficient for detection of Trak2 in 20 ug of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Storage Buffer: PBS pH7.4, 50% glycerol, 0.1% sodium azide
Conjugate: ATTO 594
Cellular Localization: Cytoplasm, Mitochondrion, Early Endosome. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Trak2 are readily searchable from our website. Different antibodies against the same target such as Trak2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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