anti-TRAP1 antibody product blog
Tags: Antibody; Monoclonal Antibody; TRAP1; anti-TRAP1 antibody;
The TRAP1 trap1 (Catalog #MBS801051) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Trap1 Antibody: Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Trap1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP).1:1000 (WB). Researchers should empirically determine the suitability of the TRAP1 trap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TRAP1 trap1 product has the following accession number(s) (GI #155722983) (NCBI Accession #NP_057376.2) (Uniprot Accession #Q12931). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Trap1 Antibody: Biotin with the following immunoassay(s):
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6. Tissue: PC3M cells. Species: Human. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Mitotracker (red) mitochondria stain. DAPI (blue) nuclear stain. (A) staining of mitochondria with MitoTracker dye; (B) staining of nuclei with DAPI; (C) staining with TRAP1 antibody; (D) tricolor composite of images A-C.)
Western Blot (WB) (Western Blot analysis of Human Heat Shocked cervical cancer cell line (HeLa) lysate showing detection of Trap1 protein using Mouse Anti-Trap1 Monoclonal Antibody, Clone Trap1-6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Trap1 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Background Info: Detects Trap1. Detects ~75kDa.
Scientific Background: The 90 kDa heat shock protein (hsp90) family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions (1, 2). Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting pre-sequence strongly implicates TRAP1 as a mitochondrial matrix protein (3).
Conjugate: Biotin. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TRAP1 are readily searchable from our website. Different antibodies against the same target such as TRAP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.