|The TSG twsg1 (Catalog #MBS690016) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse TSG (Twisted gastrulation) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s TSG (Twisted gastrulation) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Formalin.
1. Western blotting: Yes (1:500-1000) 2. IHC (Frozen): Yes (1:50-100). Researchers should empirically determine the suitability of the TSG twsg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TSG twsg1 product has the following accession number(s) (GI #12746428) (NCBI Accession #NP_075540.1) (Uniprot Accession #Q9EP52). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Twisted gastrulation (TSG) is a secreted, cysteine-rich protein that plays a role in dorsal/ventral patterning in Drosophila and Xenopus by regulating BMP signaling. TSG was originally identified in Drosophila melanogaster and shown to be required for the differentiation of the dorsal amnioserosa cells. Studies of expression and function of Twisted Gastrulation have been performed in Drosophila and Xenopus. Xenopus TSG is expressed in the ventral regions of the embryo during gastrulation, mimicking the BMP-4 expression pattern. TSG is expressed in dorsal cells of the blastoderm embryo, where there are also high levels of activity of Dpp and Screw. Vertebrate TSGs were subsequently cloned in mouse, human, zebrafish and frog. TSG acts as an agonist for BMP signaling by modulating the inhibitory actions of the BMP antagonist, Chordin/Sog, and the cleavage properties of the metalloprotease, xolloid/tolloid. The N-terminal domain of TSG can bind BMP protein directly in vitro and shows BMP antagonist activity.
Antibody Generation: This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant TSG. The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. Reconstitution: Reconstitute the antibody with 200 ul sterile PBS and the final concentration is 500 ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TSG are readily searchable from our website. Different antibodies against the same target such as TSG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.