|The Tyrosine, Phosphorylated (HRP) n/a (Catalog #MBS633503) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Tyrosine, Phosphorylated (HRP) can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot; Immunohistochemistry.
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Recommended Dilutions: ELISA: 1:2000-1:10,000
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:200-1:1000. Researchers should empirically determine the suitability of the Tyrosine, Phosphorylated (HRP) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Protein phosphorylation and dephosphorylation reactions is a key postranslational event in the modification of protein functions. Protein phosphorylation occurs at tyrosine, serine, or threonine (p-tyr, p-ser, or p-thr) residues. Many different mitogenic systems, such as the EGF, PDGF, and insulin receptor systems contain tyr/ser/thr kinase domains which autophosphorylate specific tyr, ser or thr residues upon binding of their ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated proteins. An understanding of transformation by oncogenes and mitogenic processes of growth factors requires a delineation of all potential partners involved in phosphorylation cascade. The availability of antibodies specific for p-tyr, p-thr or p-thr residues has greatly advanced the studies on role of phosphorylated proteins.
Immunogen: O-Phospho-L-tyrosine (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Tyrosine, Phosphorylated (HRP) are readily searchable from our website. Different antibodies against the same target such as Tyrosine, Phosphorylated (HRP) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.