anti-UBCH10/UBE2C antibody product blog
Tags: Antibody; Monoclonal Antibody; UBCH10/UBE2C; anti-UBCH10/UBE2C antibody;
The UBCH10/UBE2C ube2c (Catalog #MBS2401159) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-UBCH10/UBE2C Antibody (clone 9D3) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s UBCH10/UBE2C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB).IF: 10 ug/ml
IHC-P: 3-10 ug/ml. Researchers should empirically determine the suitability of the UBCH10/UBE2C ube2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UBCH10/UBE2C ube2c product has the following accession number(s) (GI #5902146) (NCBI Accession #NP_008950.1) (Uniprot Accession #O00762). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-UBCH10/UBE2C Antibody (clone 9D3) with the following immunoassay(s):
Immunohistochemistry (IHC) (Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [antibody concentration 10 ug/ml])
Western Blot (WB) (UBE2C monoclonal antibody (M01), clone 9D3 Western blot of UBE2C expression in HeLa.)
Western Blot (WB) (Western blot of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody (M01), clone 9D3.)
ELISA (EIA) (Detection limit for recombinant GST tagged UBE2C is approximately 0.03 ng/ml as a capture antibody.)
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes \'Lys-11\'- and\'Lys-48\'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating \'Lys-11\'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.
Immunogen: UBE2C (NP_008950, 70 a.a. ~ 180 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa
Conjugation: Unconjugated. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing UBCH10/UBE2C are readily searchable from our website. Different antibodies against the same target such as UBCH10/UBE2C may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.