|The UHRF1 uhrf1 (Catalog #MBS9215394) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The UHRF1 (N-terminus) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s UHRF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB ~~ 1:1000. Researchers should empirically determine the suitability of the UHRF1 uhrf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UHRF1 uhrf1 product has the following accession number(s) (GI #115430235) (NCBI Accession #NP_001041666.1) (Uniprot Accession #Q96T88). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the UHRF1 (N-terminus) Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot detection of UHRF1 in Mouse heart,293T,HelaNE and Hela cell lysates using UHRF1 mouse mAb (1:1000 diluted).Predicted band size: 90KDa.Observed band size: 90KDa.)
Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD- type zinc fingers, specifically recognizes and binds histone H3 trimethylated at \'Lys-9\' (H3K9me3) and unmethylated at \'Arg-2\' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
Antigen Source: Human. Antigen Type: Recombinant Protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing UHRF1 are readily searchable from our website. Different antibodies against the same target such as UHRF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.