anti-V5-TAG antibody product blog
Tags: Antibody; Monoclonal Antibody; V5-TAG; anti-V5-TAG antibody;
The V5-TAG n/a (Catalog #MBS219730) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s V5-TAG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB).Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/500. Researchers should empirically determine the suitability of the V5-TAG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI V5-TAG with the following immunoassay(s):
Testing Data (V5 tagged protein detected with Mouse anti V5-tag (MBS219685))
Mouse anti V5-Tag antibody, clone SV5-Pk3 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk3 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Viral. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing V5-TAG are readily searchable from our website. Different antibodies against the same target such as V5-TAG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.