|The V5 Tag n/a (Catalog #MBS588116) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s V5 Tag can be used in a range of immunoassay formats including, but not limited to, Dot Blot (Dot), ELISA (EIA), Immunoprecipitation (IP), Immunostaining (IS), Western Blot (WB).
If reconstituted in 100 ul:
Western Blot: 1:1000-1:3000. Researchers should empirically determine the suitability of the V5 Tag n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the V5 Tag Mouse Monoclonal Antibody (E10) - Purified with the following immunoassay(s):
Testing Data (Western blot analysis of HEK293 cells transfected with V5-tagged protein vector. 1:1000 (1ug/ml) Ab dilution. Untransfected (1) and transfected (2).)
Background: Epitope tags have applications in the labeling, isolation and detection of proteins using immunoblotting, Immunoprecipitation and immunostaining techniques. Epitope tags can be used, by affinity chromatography, to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Due its small size the protein\'s biochemical properties seem not to be affected by the tag protein. The V5 tag antibody can be used in many different assays that require recognition by an antibody.
Description: A highly specific and sensitive antibody against V5. The antibody was raised against GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH.
In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing V5 Tag are readily searchable from our website. Different antibodies against the same target such as V5 Tag may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.