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anti-VAMP1 antibody product blog

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Posted on 2015-08-20 11:29:41 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; VAMP1; anti-VAMP1 antibody; VAMP1/2;
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The VAMP1 vamp1 (Catalog #MBS804674) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The VAMP1/2 Antibody: Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s VAMP1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC) (paraffin embedded).
0.5-1ug/mL (WB). Researchers should empirically determine the suitability of the VAMP1 vamp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The VAMP1 vamp1 product has the following accession number(s) (GI #7657673) (NCBI Accession #NP_055046.1) (Uniprot Accession #P23763). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VAMP1/2 Antibody: Biotin with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-VAMP Monoclonal Antibody, Clone SP-11. Tissue: Cerebellum. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-VAMP Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Fibers and Neurons.)
Immunohistochemistry (IHC) VAMP1.

Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-VAMP Monoclonal Antibody, Clone SP-11. Tissue: Brain Slice. Species: Human. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-VAMP Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT. Localization: Brain neurons with lack of staining on the blood vessels. Magnification: 20x.)
Immunohistochemistry (IHC) VAMP1.

Western Blot (WB) (Western Blot analysis of Rat brain lysates showing detection of VAMP protein using Mouse Anti-VAMP Monoclonal Antibody, Clone SP-11. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-VAMP Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)
Western Blot (WB) VAMP1.

Background Info: Recognzies the human synaptic vesicle protein syanptobrevin, VAMP, Recognizes VAMP 1 and 2

Scientific Background: VAMP-1, also known as vesicle-associated membrane protein, is an 18kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils, and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane (1). Synaptobrevins, Syntakins and the 25kDa synaptosomal associated SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane (2). VAMP-1 is also one of the key proteins in the SNARE complex which is involved in regulated exocytosis (3).

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