|The VASP vasp (Catalog #MBS603306) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Vasodilator Stimulated Phosphoprotein, phosphorylated (Ser239) (VASP) (Biotin) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Vasodilator Stimulated Phosphoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS).
Suitable for use in ELISA, Western Blot, Immunocytochemistry, Flow Cytometry and Immunoprecipitation.
Dilution: ELISA: 0.05ug/ml, as reported by an independent laboratory.
Western Blot: 0.5-2ug/ml detected phosphorylated VASP in RIPA lysates from CTLL cells. CTLL cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2118 (0.5ug/ml). Proteins were visualized using a Gt xMo IgG (HRP)* and a chemiluminescence detection system.
Immunocytochemistry: An independent laboratory has reported that 3ug/ml antibody showed positive immunostaining in SNP or 8pCPT-cGMP-treated human platelets, xed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
Flow Cytometry: 2.4ug/ml using xed and permeabilized human platelets, as reported by an independent laboratory.
Immunoprecipitation: Immunoprecipitates phosphorylated VASP from vanadate-treated A431 cells, as reported by an independent laboratory. Researchers should empirically determine the suitability of the VASP vasp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VASP vasp product has the following accession number(s) (GI #4507869) (NCBI Accession #NP_003361.1) (Uniprot Accession #P50552). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF/NO/cGMP Protein kinase and cAMP/cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines; particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation. Ser 157 is a site preferred by cAMP-dependent protein kinase (PKA).
Immunogen: Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VASP are readily searchable from our website. Different antibodies against the same target such as VASP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.